| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Characterization of biofilm extracts from two marine bacteria
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| Autores: |
Passerini, Delphine
Fecamp, Florian
Marchand, Laetitia
Kolypczuk, Laetitia
Bonnetot, Sandrine
Sinquin, Corinne
Verrez-bagnis, Veronique
Hervio Heath, Dominique
Colliec Jouault, Sylvia
Delbarre Ladrat, Christine
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| Data: |
2019-11
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| Ano: |
2019
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| Palavras-chave: |
Vibrio
Biofilm
Polysaccharide
Quorum-sensing
Activity
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| Resumo: |
In the marine environment, biofilm formation is an important lifestyle for microorganisms. A biofilm is comprised of cells embedded in an extracellular matrix that holds them close together and keeps the biofilm attached to the colonized surface. This predominant lifestyle and its main regulation pathway, namely quorum-sensing (QS), have been shown to induce specific bioactive metabolites. In this study, we investigated the biofilm formation by two marine bacteria belonging to the Vibrio species to discover potentially innovative bioactive compounds. We proposed a protocol to isolate biofilm extracts, to analyze their biochemical composition, and to compare them to planktonic cell extracts. Cells were grown attached to a plastic surface; extracts were prepared in water, NaOH, or in ethyl acetate and analyzed. Extracellular matrix components featured carbohydrates, proteins, lipids, and low amount of DNA. Carbohydrates appeared to be the main constituent of biofilm but also of the planktonic cell supernatant. Moreover, antimicrobial and QS-signaling activities were evidenced in extracts.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00592/70381/68461.pdf
https://archimer.ifremer.fr/doc/00592/70381/68488.pdf
DOI:10.3390/app9224971
https://archimer.ifremer.fr/doc/00592/70381/
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| Editor: |
MDPI
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| Formato: |
application/pdf
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| Fonte: |
Applied Sciences-basel (2076-3417) (MDPI), 2019-11 , Vol. 9 , N. 22 , P. 4971 (20p.)
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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